Which optimization algorithm is most efficient for fitting a regression model using training dataset?

The goal of regression model is either to minimize the prediction error or to maximize a classification accuracy depending on the type of the model. Is there any optimization algorithm that is...

10 February 2021 4,128 3 View

Any end to end machine learning project using predictive maintenance on production line to locate faulty parts and forecast the faults?

I am writing my master thesis and my tasks are: To locate faulty stations/features which lead to product failure at test time. Forecast failure to prevent it sometime before it occurs. recommend...

13 December 2020 3,688 3 View

How to construct complete 3D structure of a protein from two different PDB files containing two separate portions of the same protein?

Suppose, I want to model a protein's structure (containing total of 350 residues) for which there is no complete experimental structure available. However, I got two different PDB files each...

13 November 2020 9,110 3 View

Qiagen DNeasy vs QIAquick spin column?

Dear Fellows I wanted to confirm if I can use spin columns of QIAquick PCR purification kits for DNA extraction. We finished our DNeasy columns but still have reagents. And we have extra QIAquick...

03 November 2020 6,960 3 View

How to reduce number of mesh cells for non-uniform 3D objects?

I want to reduce number of mesh cells in my 3D non uniform model

12 October 2020 9,522 3 View

Is there any minimum limit of outside projections of shell side nozzles of heat exchangers in DNV standards?

Is there any DNV clause for shell side nozzles' projections of shell and tube heat exchanger. I want to keep the outside projection of the shell side inlet and outlet nozzles to 3" with 1" bore on...

29 September 2020 6,880 3 View

Hello, what method/model is suitable for estimating three dependent vars. influenced by other independent vars. in a time series analysis?

The dependent variable (petroleum security) is sub-divided into three categories (oil price, oil supply and oil reserve), and are determined by currency internationalization. So, which...

19 September 2020 8,149 1 View

How can I eliminate discontinuities in the end-effector plot of an industrial manipulator?

I am trying to solve a dynamic problem for a 3 DOF industrial manipulator and I keep getting funny end-effector plots for the first joint (see the figure). I have tried using the alternate...

21 June 2020 7,931 15 View

How can i obtain Parameters of Chinese trunk track irregularity power spectral density function using Matlab?

Please I need explanation on how to determine the parameters (A, B, C, D, E, F, G) of the Chinese trunk track irregularity power spectral density function from experimental data using the...

28 March 2020 8,752 3 View

How to validate ligand parameters ?

I wanted to perform a molecular dynamics simulation of a protein-ligand complex in Gromacs using the CHARMM36 force field. When generating the ligand topology file using CGenFF...

05 March 2020 2,041 6 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

How to measure 50uL of cells?

Hello, I am planning to do a transformation, and on the protocol it says "Scrape a 50 ml volume of yeast from the YPAD plate using a sterile inoculating loop or sterile toothpick". But I can...

14 February 2021 6,247 3 View

Cells not growing in large scale protein expression?

I am using KRX cell. I have grown my overnight cultures at 37 degrees. When I inoculate 1L TB media, my cells do not grow. I have also tried using 25 degree incubation of overnight cultures and...

10 February 2021 3,871 3 View

Can I use the Chlorophyll stability index (CSI) to calculate the stress potential of cyanobacteria that are under stress?

I want to calculate the stress potential of cyanobacterial cells that are inoculated with antagonistic bacteria. My results showed that there is a drastic reduction in the chlorophyll content of...

23 January 2021 331 3 View

How to revive bacteria from glycerol stock?

19 January 2021 7,025 4 View

How can I inject a special bacteria to a laboratory mouse?

Hi, I want to inject (inoculate) a special bacteria to a laboratory mouse. Could you please guide me if you know any useful method?!

15 December 2020 1,430 5 View

Bacteria growing well on nutrient broth but not on NA plates. What could be reason? How can it be resolved?

I am facing a problem. I have isolated few bacteria from soil and preserved them in glycerol stock (50 % glycerol v/v). Now when I am inoculating these cultures in nutrient broth and incubating...

06 December 2020 3,577 6 View

Can anyone help with SDS-PAGE problems?

i'm working on protein evaluation in flour of wheat and others legumes.....I'm using SDS-PAGE. However those days (about two weeks ago) i foudd nothing in the gels (no bands), i dont know what's...

11 November 2020 2,330 3 View

Why am I not getting high yield plasmid?

I am trying to isolate many plasmids for a project (ranging from 6.6Kb to 6.2Kb), and the plasmids have a pUC1 ori (high copy number from what I can tell), and Amp resistance. I am using...

09 November 2020 2,949 3 View

What volume of liquid yeast would be ideal to inoculate into a liquid Sabouraud media?

I am working on enhancing brewers yeast viability, and I am using Liquid Sabouraud media and liquid Saccharomyces cerevisiae. Initially, I made a single serial dilution and inoculated 1ml of the...

24 October 2020 554 7 View