After the addition of nuclease-free water in DNA samples, After how long we can run gel electrophoresis ?

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Paul Rutland

It should be stable for many months at 4c but storage of dna is better in TE because the tris buffers the sample alkaline so stopping depurination and the edta will stop nuclease activity by chelating divalent cofactors like magnesium essential for nuclease activity

Fatemeh Yaghoobiadeh

If you do not want to run dna immediately, it is better to store it in -20 and avoid multiple freeze-thaw cycles.

Abid Rather

After adding nuclease-free water to DNA samples, you can generally run gel electrophoresis almost immediately.