Whether it is compulsory to carry out in-situ water sampling and laboratory-based analysis for a water quality study based on remote sensing?

A study assessed the water quality status using a few parameters such as Chlorophyll a, BOD, COD, TSS and etc to determine the pollution level of water bodies using remote sensing techniques....

19 September 2020 4,839 3 View

How successful use of the DPSIR framework to study the sustainability of wetland ecosystems?

The developed countries have mostly used different approaches, frameworks to achieve the targets of SDGs (Edward, et al., 2007) while restoring the freshwater ecosystems in more productive aspect...

16 September 2020 1,795 7 View

What are the suitable methods to extract water quality data from Landsat 8 satellite images?

There are various methods/ algorithms used to extract water quality data from satellite images. Most of them are applied for large water bodies. I am looking for a suitable method to extract water...

29 August 2020 5,204 3 View

Why values not changing in terminal and not simulating correctly?

When customizing the transmission ranges of nodes in tcl file, NS2 taking lowest transmission range in simulation. How can I change the value into highest (1000m or 2050m)? #terminal...

17 March 2020 9,257 2 View

Hi this is problem on HEC HMS software

i am doing a research base on HEC RAS and HEC HMS softwares. but in HEC HMS there can not be changed date of the time series data manger. i have attached some photograph of that. so can anyone...

23 May 2017 647 1 View

How to derive confidence intervals for price elasticities calculated at sample means through SUR system?

08 November 2016 6,177 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View