Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Please. Could you help be in implementing Fractal Discrete Cosine Transform algorithm in Matlab?

I do need the Matlab code of Fractal Discrete Cosine Transform (FDCT). Can anyone who has already implement this code, help me with the implementation of this transform?

24 February 2021 5,602 2 View

How can I homogenize this data to make a meta-analysis?

Hi, I am doing a review and hopefully meta-analysis for my Master thesis but I have the following problems: - Some studies only give the difference between end trial and baseline data and other...

22 February 2021 1,115 4 View

Research Gate Does it help researches to publicize their scientific work ?

In my opinion, the RG site is useful in solving scientific problems and shows the researcher's ability. Besides, the separation of the H-index from the self-citations and the overall index is an...

22 February 2021 3,894 6 View

Monte Carlo simulation?

Hi, i am conducting a Monte Carlo simulation for my new distribution but the MSE values are increasing while the sample size increase, does any experience this issue before and what should i do...

22 February 2021 3,680 4 View

Which is the best software or programming language (user friendly) for analysing data?

I have a basic knowledge on Python, R and MATLAB in analysing data. Among those, which is the best and user friendly? Which one should I learn deeply to analyse data?N:B: I want to learn data...

20 February 2021 3,950 15 View

What is the reason film thickness increased?

what is the reason film thickness increased when increased doping concentrations give some reasons The measured film thickness is 156, 282, 394, 548 and 681 nm for 0, 1.5, 2.5, 3.5, and 4.5 wt....

17 February 2021 8,987 5 View

Is There are any idea to achieve H NMR signals for swollen compound ??

the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble any suggestion, please

16 February 2021 8,801 3 View

Is there any chemical safe for sea environment, but could be used for repelling planktons and microorganisms?

As an example of Julia Child, whose first receipe was shark repelent, my team and I are looking for some chemicals which wont harm planktons but will help keep planktons from our robot in sea.

20 December 2020 9,616 4 View

I am looking for advice. How to prepare bacterial cells for AFM images (live and fixed on glass slide)?

We have a new AFM with a capability to achive high resolution images (up to 50nm). The AFM tips can scan the surface of the object and as well, can penetrate into the object. We also have the...

07 November 2020 2,813 7 View

Why do I receive the sae mesage several times?

26 October 2020 409 1 View

I need to do a research project on how human action impacts zooplankton. Any ideas from brilliant minds?

I need to do a research project on how human action (e.g. climate change, ocean acidification, plastic, pollution, HAB, etc), impacts zooplankton and the zooplankton response. I'm struggling on...

23 March 2020 2,081 5 View

If an animal feeds mainly on algae but also eats cyanobacteria and insects, which group does this animal belong to; herbivore or omnivore?

I wonder if a fish (Random sp.) feeds mostly on plankton but also consumes cyanobacteria and some insects (Chironomidae, Simuliidae) at much lower rates, can we classify them as omnivore?

03 March 2020 8,177 4 View

If an animal feeds mainly on algae but also eats cyanobacteria and insects, which group does this animal belong to; herbivore or omnivore?

I wonder if a fish (Random sp.) feeds mostly on plankton but also consumes cyanobacteria and some insects (Chironomidae, Simuliidae) at much lower rates, can we classify them as omnivore?

02 March 2020 1,300 3 View

Is there a lab that determines plankton sample energy contents?

Dear all, I was wondering if anybody knows of a lab that analyses foreign powderised plankton samples via bomb-calorimetry for energy density? I got some energy values that are one order of...

09 December 2019 6,029 3 View

How do I best determine the energy content of gelatinous zooplankton?

Hi! I am trying to measure the energy content of various gelatinous zooplankton organisms using a semi-micro bomb-calorimeter. Because of the low expected energy content, I am mixing my sample...

19 November 2019 2,566 4 View

Any mobile App or software to identify Plankton from microscopic photos?

Are there any mobile applications or software to identify plankton from microscopic photos of pond water? This is needed for a participatory training programme.

09 October 2019 4,097 3 View

Which microparticles to use as food competitor for zooplankton?

Hi everyone, We are looking for non-toxic microparticles that could be handled and ingested by zooplankton. We would like to use them as an external perturbation impacting the functional response...

06 October 2019 2,877 1 View