Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Please. Could you help be in implementing Fractal Discrete Cosine Transform algorithm in Matlab?

I do need the Matlab code of Fractal Discrete Cosine Transform (FDCT). Can anyone who has already implement this code, help me with the implementation of this transform?

24 February 2021 5,602 2 View

How can I homogenize this data to make a meta-analysis?

Hi, I am doing a review and hopefully meta-analysis for my Master thesis but I have the following problems: - Some studies only give the difference between end trial and baseline data and other...

22 February 2021 1,115 4 View

Research Gate Does it help researches to publicize their scientific work ?

In my opinion, the RG site is useful in solving scientific problems and shows the researcher's ability. Besides, the separation of the H-index from the self-citations and the overall index is an...

22 February 2021 3,894 6 View

Monte Carlo simulation?

Hi, i am conducting a Monte Carlo simulation for my new distribution but the MSE values are increasing while the sample size increase, does any experience this issue before and what should i do...

22 February 2021 3,680 4 View

Which is the best software or programming language (user friendly) for analysing data?

I have a basic knowledge on Python, R and MATLAB in analysing data. Among those, which is the best and user friendly? Which one should I learn deeply to analyse data?N:B: I want to learn data...

20 February 2021 3,950 15 View

What is the reason film thickness increased?

what is the reason film thickness increased when increased doping concentrations give some reasons The measured film thickness is 156, 282, 394, 548 and 681 nm for 0, 1.5, 2.5, 3.5, and 4.5 wt....

17 February 2021 8,987 5 View

Is There are any idea to achieve H NMR signals for swollen compound ??

the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble any suggestion, please

16 February 2021 8,801 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Where would? can? positive charge(vacancy) be when grounded?

Hello I plasma(H2N2) treated Al2O3 plate (edge is connected to ground) and wondered if oxygen vacancy migration on the plate can occurs as electrons escape through edge. Or whatever positive...

15 February 2021 8,118 1 View

ZnS oxidizes to ZnO!!why??

when I put my ZnS solution in the chemical bath, the ZnS oxidizes so there are ZnO peaks appearing because of the reaction with the oxygen in the air, despite the fact that I close the beaker...

14 February 2021 5,778 9 View

How to prepare OH- terminated Si Wafer?

Hi, Folks. I am working on the ALD project to deposit Al2O3 on the Si wafer from TMA and Water. I have struggled with the preparation of OH- terminated Si wafer. I have both Si(1 0 0) and...

08 February 2021 8,792 2 View

Is it possible to have a diffusion of oxygen from the glass substrate (SiO2) into the absorbent layer during annealing process?

02 February 2021 382 7 View

How to do ORR test with pine(Which means RDE or RRDE electrode)?

I want to do LSV ,CV test of ORR(Oxygen reduction reaction). But our laboratory don't have the RDE and RRDE electode. And I was wondering if I can do the tests by magnetic stiring or mechanical...

14 January 2021 1,869 2 View

Can I use saponin to permeabilize cell to measure mitochondrial respiration?

Saponin and digitonin both can permeabilize cells. However, based on many respiration assay protocols I read, saponin is used for tissue permeabilization and digitonin is used for cell...

11 January 2021 2,151 3 View

How is oxygen vacancy formed in Tio2 and help with anatase to rutile transformation?

I am unable to visualize the basic formation of Oxygen vacancies in Tio2 as sometimes information is a bit scattered. Can anyone help me with how these point defects are formed and what prompts ?...

08 January 2021 2,017 4 View

Can you give me some Information about the generation of ROS in marine surface water?

Reactive oxygen species is a very new term i think

23 December 2020 8,416 4 View

How to calculate the gas composition for a heat treatment where a very low oxygen partial pressure is needed?

I'm interested in a selective oxidation in an austenitic stainess steel so that Chromia rather then ferrous oxide is formed on top of my metal. I know I should have a value of pO2 around...

16 December 2020 8,541 7 View