Hi,
I am currently attempting to calculate the Encapsulation efficiency of a nanoparticle formulation. I added an initial 25 mg amount of the drug (donepezil) and after centrifugation of the formed nanoparticles tried to read the absorbance of the supernatant via UV spectroscopy. While I was expecting an absorbance unit below 0.482 (which corresponds to 25 mg of donepezil according to my calibration curve), I got a 0.629 AU. I am certain that my calibration equation is accurate, and that experimental steps are carried out carefully. However, the supernatant contains traces of Glutradialdehyde and gelatin which were used in the nanoparticle formation. What can I do to get the absorbance of Donepezil alone? ( the wavelength is 316 nm)
Dear Mohammad Moghaddasi
Check your cuvette. Also, your sample my not be pure enough after centrifugation.
Dear M. R. Mozafari ,
Thank you for your response. I've made sure to wash the cuvette completely before analysis, and it has generated reliable data before. I suspect that the presence of gelatin and Gluteradialdeydhe in the supernatant is also contributing to the absorbance at 316 nm, thus giving a higher AU. In this case, I think HPLC would be the only option to get the amount of DP.
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