hello, I have attached a file of my gel, I ran protein sample on gradient gel,
80V, and I can see where there are protein samples, I have a weird shape of gel while running
anyone please help
How do you prepare your samples? I mean, after add SDS-PAGE and sample buffer to the samples you put the samples in the thermal cycler to 95 degrees for 10 minutes ?
hey, thanks for answering, Yes I put my samples in the thermal cycler to 95 degrees but for 5 minutes.
From the picture, I see some disturbance of the dye front. In my experience, this can be caused by the presence of a large amount of lipid in the sample, such as would occur in a sample prepared from a purified membrane preparation.
I don't know, but i took less amount of protein taking from the lysate, and gel ran perfectly.
Hi Amani,
We have the same problem too and that usually happens when we have high concentration of detergent in our samples. It doesn't affect our protein bands though.
hey xiaojun yeah it doesn't affect it, but for the first time to get such gel was scary
but if i load more than 40ug of proteins in the gel i get such shape,
but now i load less amount and gel runs perfectly fine, but as you adjusted it does not affect protein bands on detection
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