RNA isolation from mouse skeletal muscle tissues, what's the best way to execute this?

I am trying to isolate RNA from mouse skeletal muscle but I am not able to get a good 260/230 reading. I am using Qiagen RNeasy kit. I would appreciate any suggestion on isolating pure RNA from...

13 March 2019 6,875 4 View

How to start on SELEX and aptamer related work?

Hi. I am new to this field and I would like to learn more about aptamers and and work on their diagnostic applications. Would very much appreciate if anyone could give me inputs on the things to...

05 December 2018 8,653 2 View

Which solvent i can used for cerium oxide nanoparticles sample for FTIR,UV and SEM analysis?

I have prepared Cerium oxide nanoparticles using co-precipitation method.Now i want to characterized it using SEM and FTIR.Can anyone suggest me which solvent i can use to prepare sample for...

07 January 2018 498 7 View

Please suggest is there any other method to remove palladium acetate from product mixture apart from using column chromatography?

Chepuri Venkata Ramana

05 April 2017 1,364 3 View

Which simulation tool is better for implementing a secure routing protocol in Wireless Sensor Networks Ns3 or Omnet++?

Which simulation tool is better for implementing a secure routing protocol in Wireless Sensor Networks Ns3 or Omnet++?

31 March 2017 3,428 9 View

Does anyone know any Adhesive Epoxies with good electrical and thermal insulation properties?

I current fabricating a THW Cell in order to experimentally find the thermal conductivity of certain nanofluids. I need the adhesive epoxy to suspend the platinum hot wire as well as other copper...

05 September 2016 8,576 4 View

What role does "diffusion term" play while solving a scalar transport equation on a fully converged flow?

I am trying to find the time a constituent (a powdered solid) would take to get mixed in the mixture. For this i have solved the flow separately and then on the fully converged flow i am trying to...

19 July 2016 5,601 6 View

Can a drug have two different IC50 values in two different cell lines. Please guide?

Can a drug have two different IC50 values in two different cell lines. Please guide?

03 June 2016 5,749 6 View

What are the different methods for finding the thermal conductivity of fluids and where can I find explanations to how these methods work?

28 January 2016 4,138 2 View

Yeast cells undergo salvage pathway and if there is a variation in physiological factors, does it affect the production of adenine?

nucleic acid production in salvage pathway

24 August 2014 1,162 2 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View