Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?
Hey Duy,
The probe is not specific for any ROS subspecies and diffusable, therefore you can measure total cellular ROS with this probe.
If you like, please have a look at our recent review paper:
"Functions of ROS in macrophages and antimicrobial immunity".
Article Functions of ROS in Macrophages and Antimicrobial Immunity
In this review, we give an introduction to ROS and their sources in macrophages, summarize the versatile roles of ROS in direct and indirect antimicrobial immune defense and provide an overview of commonly used ROS probes, ROS source inhibitors and ROS scavengers (also the difference between ROS scavengers and antioxidants, which are not synonymous, is explained).
Functions of ROS in Macrophages and Antimicrobial Immunity
February 2021, Antioxidants 10(2):313
DOI: https://doi.org/10.3390/antiox10020313
All the best and stay healthy,
Marc
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