I have prepared Cerium oxide nanoparticles using co-precipitation method.Now i want to characterized it using SEM and FTIR.Can anyone suggest me which solvent i can use to prepare sample for SEM,FTIR and UV analysis.
For SEM and FT-IR, no need to prepare aqueous suspensions, just dry powder is enough. If you have the possibility to do UV-Vis DRS (diffused reflectance spectroscopy), then again no need of aqueous solvent. You can measure UV-DRS directly with powder.
If not then you need to take minute amount (I used to disperse powder with 0.05 mg/ml concentration in DI Water) followed by sonication to make a colloidal solution and then take UV-Vis absorption.
Aqueous solutions of Nanoparticles can be used for analysis of Nanoparticles. As far as FT-IR and SEM is concerned, dried/powdered nanoparticles followed by sputtering can be directly processed for FT-IR and SEM based characterization.
For SEM and FT-IR, no need to prepare aqueous suspensions, just dry powder is enough. If you have the possibility to do UV-Vis DRS (diffused reflectance spectroscopy), then again no need of aqueous solvent. You can measure UV-DRS directly with powder.
If not then you need to take minute amount (I used to disperse powder with 0.05 mg/ml concentration in DI Water) followed by sonication to make a colloidal solution and then take UV-Vis absorption.
SEM: Dry powder on carbon sticky tape
FTIR: Dry powder or colloidal suspension in water or IPA. Make sure the stretching frequency of water or IPA doesn’t overlap with that of Cerium oxide before choosing dispersing solvent. I would recommend using it as a dry powder.
UV: CeO2, like most metal oxides is hard to dissolve except using acid (HCl works fine). Using colloidal dispersions in DI water should be okay. UV diffuse reflectance can be done using powder sample, but you will need a large amount of sample.
Dear Deepak Kaala,
Luckily Your Material (CeO2-NPs) is well known to me. In our previous investigations, we have found that dispersion of CeO2-NPs in deionized water (DI) will give ideal results. Especially for UV and FTIR testing. To develop further understanding I would recommend you to read material and Method section of following two Research Articles.
1- Green-synthesised cerium oxide nanostructures (CeO2-NS) show excellent biocompatibility for phyto-cultures as compared to silver nanostructures (Ag-NS).
2- Antimicrobial potential of green synthesized CeO2 nanoparticles from Olea europaea leaf extract.
Hope this will supplement your concern.
-All the Best-
For SEM & FTIR analysis You need no solvent. These physicochemical analysis can be done in dry powdered form. For UV-Vis analysis dispersion of prepared nanoparticles in deionised water can be form by sonication.
Wish you all the best
Regards
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