02 February 2015 1 3K Report

Mobile system was Acetone: Petroleum ether: Methanol

Please help in this TLC anamoly. please see the image attached.

No spray have been done on the TLC shown

Also I want to interpret the TLC for the presence of lutein, so what standard can i use. Sigma standard lutien are  too costly to be procured....what are the alternatives..

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