I have extracted marigold oleoresin using petroleum ether and have confirmed the presence of lutein but want to quantify it. And also for nanoencapsulation purpose how to seperate pure lutein from that oleoresin? Please help in this regard.......
A chemical free two-stage process and the products for the extraction of lutein diester from marigold meal comprises dissolving lutein diester and lipophilic material from marigold meal in supercritical fluid carbon dioxide at certain temperatures and pressures, and separating out a high quality lutein diester extract(FIG. 2). The lutein diester extracted is utilized for human nutraceutical and food supplement applications.
2. Process for isolation, purification, and recrystallization of lutein from saponified marigold oleoresin and uses thereof
US 5382714 A
ABSTRACT
A method of isolating, purifying and recrystallizing substantially pure lutein, preferably from saponified marigold oleoresin in its pure free form, apart from chemical impurities and other carotenoids. Lutein may be used as an analytical standard or in cancer prevention trials and as a safe and effective color additive for human food.