Hi all I have a full length (FL) sequence gene and few constructs of the gene to produce a functional protein.. which I would purify if all goes well.
Firstly, I did normal cloning of my FL gene and its constructs i.e. Molecular cloning of them using Pcold vector TF and Prs2.. It seemed positive till now and I have send the clones that were positive for sanger sequencing (did mini prep etc for the same using Kit) awaiting results.
Now the second part which is that I tried LIC and ran gels I obtained faint bands which was of the primers and not the desired sequence.
Tried with optimizing the PCR by adding DMSO.
Please suggest how could solve this problem.
Regards,
Ratish Raman