Hi all I have a full length (FL) sequence gene and few constructs of the gene  to produce a functional protein.. which I would purify if all goes well.

Firstly, I did normal cloning of my FL gene and its constructs i.e. Molecular cloning of them using Pcold vector  TF and Prs2.. It seemed positive till now  and I have send the clones that were positive  for sanger sequencing (did mini prep etc for the same using Kit) awaiting results.

Now the second part which is that I tried LIC and ran gels I obtained faint bands  which was of the primers and not the desired sequence.

Tried with optimizing the PCR by adding DMSO.

Please suggest how could solve this problem.

Regards,

Ratish Raman

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