Ratish Raman

4 Questions 8 Answers 0 Followers

Questions related from Ratish Raman

Hi guys ... I wish you check whether mayers hematoxylin and myers acid hematoxylin are one and the same or different ?

please advice... as I would be performing histological staining using alcian blue Haematoxylin staining on zebrafish cryosections to look at cartilage element or strs.

14 April 2021 7,706 3 View

How can I improve the yield of PCR purified products using QIAGEN or is there any other method?

I will be procedding with rna probe designing.but before that I am performing pcr to create antisense and sense products and then purifying them. I have performed PCR and using QIAGEN PCR...

30 September 2015 2,741 4 View

How will I know if the PCR product is oriented (Fwd. or reverse) in the sequence data?

I have performed cloning for the gene of interest using pcrII TOPO vector. After the slection steps and innoculation steps I isolated the plasmids using QIAGEN kit and then measured using...

22 September 2015 321 3 View

Why did LIC for my full length gene and constructs not work?

Hi all I have a full length (FL) sequence gene and few constructs of the gene to produce a functional protein.. which I would purify if all goes well. Firstly, I did normal cloning of my FL gene...

05 August 2014 9,106 4 View