Andreas M Kist

2 Questions 5 Answers 0 Followers

Questions related from Andreas M Kist

When eluting from Magnetic Protein A/G beads with SDS sample buffer, do I have to use DTT?

I'd like to investigate if my protein of interest is covalently bound to the protein which was caught by the antibody. Can I leave the elution buffer (which is my SDS sample buffer) without DTT?...

01 October 2013 1,012 3 View

Can I use HisProbe-HRP with milk instead of BSA?

I bought the HisProbe-HRP system from Thermo. First experiments showed very very nice bands and it is much more sensitive than anti-6xHis-Antibodys. But it is recommended using BSA as a blocking...

08 September 2013 7,673 3 View