I am looking for advice from experienced individuals regarding the optimal conditions for plasmid electroporation in A375 cells. Would anyone be willing to share their expertise on this matter?
Additionally, I attempted electroporation using 140, 180, and 240 volts for both 70 ms and 35 ms, but unfortunately, the cells did not survive. Any suggestions on how to improve the outcomes would be greatly appreciated."
Are you able to try the Lonza Nucleoffector. I've found it leads to less cell death and improved transfection efficiency.
https://bioscience.lonza.com/lonza_bs/GB/en/Transfection/p/000000000000191667/Cell-Line-Nucleofector™-Kit-V
Have you also considered lipofection?
Martin O'Reilly
thank you for your reply.
our lab just have BTX electroporator.
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