Why centrifugation is required at 3000 rpm when performing reducing power assay for medicinal plants ? ?

More Mohini Tayade's questions See All

How to dissolve selenium nanoparticles powder?

After successfully synthesizing selenium nanoparticles from plants using water my next objective is to assess their antioxidant activity. However, I encountered an issue during the drying process...

18 January 2024 3,156 3 View

Why there is need to used catalyst during nanoparticles synthesis?

Good evening everyone I am working on green synthesis of selenium nanoparticles from leaf extract. The research article mentions that during selenium nanoparticle synthesis, ascorbic acid should...

24 September 2023 4,015 2 View

Why ascorbic acid used as standard for ABTS assay?

ABTS can dissolved in both water and organic solvent. for ABTS assay we can used trolox catechin and ascorbic acid as standard, but mostly ascorbic acid used as standard, and why. why ABTS assy...

09 July 2023 3,455 3 View

How to calculate plant extract concentration (that we want to use to perform experiment like 1mg/ml-5mg/ml) from mother plant extract?

i want to use 1mg/ml to 5mg/ml concnetration for antioxidant assay. my total drug concentration (mother drug ) is 12mg. how to calculate 1-5mg/ml con. from mother drug if total reaction volume is...

30 January 2023 9,656 2 View

How to calculate the standard deviation for total phenolic content?

i want to add my data on abstract for my research paper so how to calculate and plot the values in that and also from which value we have to calcualte the standard deviation

22 December 2022 7,017 1 View

How to calculate resolution from if retention factor and plate number only given?

coumn length is 50 cm and unretained marker takes 2 min to be detected. calculte the resolution for the peaks (A-B, B-C) how long must the column be in order to have baseline resolution? assume...

24 November 2022 8,732 1 View

How to calculate IC50 value for nitric oxide scavening assay?

i have done triplicate experiment for nitric oxide assay and i take 25-225ug/ml concnetration. and i got 3 absorbance value for each concnetration. but dont understand how to calculate IC 50 value...

01 September 2022 5,792 1 View

Why sometimes control not showing colour during experiment?

i am doing metal chelation assay for that i used iron (II) Chloride tetrahydrate and ferrozine. i take 0.1 ml iron (II) chloride tetrahydrate in test tube and added 0.2ml ferrozine as a control...

18 July 2022 3,301 0 View

I am doing hydrogen peroxide scavenging activity of plant extract . I am using 1/50 dilution of extract so do I need to take pure or diluted extract?

I am using 1/50 dilution of (50-250 microgram concentration) and 3 ml of H2O2 but absorbance of sample continuously increasing. Or some time negative absorbance also getting I don't understand...

15 April 2022 6,221 3 View

Why does callus of leaf tissues in somatic embryo genesis forms on midrib and small veins in dicots?

in coffee I am observing that primary callus is mainly formed on the midrib section and the lateral veins present on the leaf. the callus doesn't form on all the 4 sides of the leaves except the...

09 February 2022 8,593 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

Culture plates for PBMC (non-adherent). For infection?

Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...

24 July 2024 2,158 2 View

Chinaza Godswill Awuchi

Check https://faqs.tips/post/why-do-we-centrifuge-at-3000-rpm-for-10min-when-performing-reducing-power-assay.html

https://www.hindawi.com/journals/tswj/2014/279451/

Also check https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-015-1228-3

https://www.sciencedirect.com/science/article/pii/S1319016412000357

Kiprotich Kiptum

Check on this paper

https://bibliotecadigital.ipb.pt/bitstream/10198/14416/1/Bouranen_Amira.pdf