Mohini Tayade

9 Questions 3 Answers 0 Followers

Questions related from Mohini Tayade

How to calculate plant extract concentration (that we want to use to perform experiment like 1mg/ml-5mg/ml) from mother plant extract?

i want to use 1mg/ml to 5mg/ml concnetration for antioxidant assay. my total drug concentration (mother drug ) is 12mg. how to calculate 1-5mg/ml con. from mother drug if total reaction volume is...

31 January 2023 9,617 2 View

How to calculate the standard deviation for total phenolic content?

i want to add my data on abstract for my research paper so how to calculate and plot the values in that and also from which value we have to calcualte the standard deviation

23 December 2022 6,998 1 View

How to calculate resolution from if retention factor and plate number only given?

coumn length is 50 cm and unretained marker takes 2 min to be detected. calculte the resolution for the peaks (A-B, B-C) how long must the column be in order to have baseline resolution? assume...

25 November 2022 8,711 1 View

How to calculate IC50 value for nitric oxide scavening assay?

i have done triplicate experiment for nitric oxide assay and i take 25-225ug/ml concnetration. and i got 3 absorbance value for each concnetration. but dont understand how to calculate IC 50 value...

02 September 2022 5,765 1 View

Why sometimes control not showing colour during experiment?

i am doing metal chelation assay for that i used iron (II) Chloride tetrahydrate and ferrozine. i take 0.1 ml iron (II) chloride tetrahydrate in test tube and added 0.2ml ferrozine as a control...

19 July 2022 3,277 0 View

I am doing hydrogen peroxide scavenging activity of plant extract . I am using 1/50 dilution of extract so do I need to take pure or diluted extract?

I am using 1/50 dilution of (50-250 microgram concentration) and 3 ml of H2O2 but absorbance of sample continuously increasing. Or some time negative absorbance also getting I don't understand...

16 April 2022 6,203 3 View

How to prepare 43mM hydrogen peroxide from 30% hydrogen peroxide chemical ?

Give me details calculation.

04 January 2022 8,331 1 View

Why centrifugation is required at 3000 rpm when performing reducing power assay for medicinal plants ? ?

what is the aim of centrifugation in reducing power assay and is centrifugation required for a standard also [ Ascorbic acid]

01 January 2022 5,890 3 View

Why use dilute extract for DPPH assay instead of using direct sample ?

if we use a direct sample to test the DPPH scavenging activity of the sample what will happen and why we need to dilute extract before performing the DPPH assay

16 December 2021 9,694 6 View