I am using 1/50 dilution of (50-250 microgram concentration) and 3 ml of H2O2 but absorbance of sample continuously increasing. Or some time negative absorbance also getting I don't understand what is the result behind it.
Thank you in advance
Ayad Palani
Dear Mohini ,
There are many issues that you need to check.
1-did you use quartz cell?
2- did you use dry or fresh sample?
however, the negative absorbance indicates that the sample does not contain enough enzyme that mean that you need to decrease the dilution ratio
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Hathama Razooki Hasan
To be sure that there is an activity you should try different volumes of the diluted as well as of the undiluted extract.
Good luck.
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Hiralal Merwade
H2O2 used for this dilution is more proportianately reduce the vol f H2O2
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