I performed native page electrophoresis on three single-strand DNA. The concentration of the page was 12%.
The relative molecular masses of the three DNA are10300 7884 and 7449.
The DNA 1 are modified with cholesterol, DNA 2 are modified with PC linker, and DNA 3 are modified with TAMRA.
The quality of the three DNA is all 100ng. I could only see the band of strand 3, but not the band of 1 and 2.
I can't figure out why this phenomenon occurs. Could any one give me some advice? If you can answer my question, I would be very grateful.