Why are two (non-RNA) bands appearing in my 1% agarose gel of gDNA?

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Why are my bands weird in this Agarose gel?

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Craig Silver

Similar problem encountered (and suggestions/help) here:

https://www.researchgate.net/post/I_am_getting_two_bands_on_agarose_gel_when_running_genomic_DNA_of_yeast-can_anyone_help

Iskander Madhi

Hi, you have two possible explanations. First one band is your specific band and the other is the primers band. The second is your some deficiencies during the execution of your DNA extraction. I use myself this kit and at first I got some nonspecific bands because of improper execution. Good luck

Noha Tharwat Abou El-khier

This may be a mechanical shearing of genomic DNA

Hassan Nima

agree with @ Noha Tharwat Abou El-khier

regards