Iskander Madhi

10 Questions 43 Answers 0 Followers

Questions related from Iskander Madhi

Can anyone help me with BD Accuri C6 Software?

I need to figure out a way how to modify the graphs. By modifiying the graphs I mean changing the frame or the style that makes the line of each graph, also, I want to change the filling of each...

14 August 2019 8,546 2 View

Does the hepatoma cell line, HLCZ01, really support HBV and HCV entire life cycle?

Hello everyone, I am really intrested on the mechanisms of virus entry and replication of HBV and HCV in different hepatoma cell lines. I heard about the new established hepatoma cell line,...

12 May 2016 4,213 1 View

HBx detection via Western Blot?

Hello everyone, I am trying to detect HBx protein from Hepatitis B Virus through Western Blot. Knowing that the molecluar size of this protein is 17kD can anyone give me an indication about the...

27 April 2016 6,670 2 View

Core and HBx proteins western blot detection?

Hello everyone, I need to detect core protein (25kD) for HCV and HBx (17kD) from HBV by western blot. I already detected but the band are still not as clear as I want. I used a 12% SDS gel,...

18 April 2016 6,926 4 View

Hbx snd core protein western blot detection?

Hello everyone, I am trying to detect hbx and core protein with western blot, is it better to use a 10% or 12% Sds-polyacrylamide gel? Thank you in advance

05 April 2016 3,042 3 View

HCV and HBV real-time PCR quantification?

Hello everyone, can someone tell me the exact differences in the different parameters (florescent dye, number of standards, quantity of the template...) of the real-time PCR for the HBV and HCV...

31 March 2016 7,071 1 View

Can anyone share methods for HCV RNA detection with PCR?

Can anyone shear with me the (RT-PCR or PCR) conditions for a good detection for the HCV virus. Thank you in advance.

18 March 2016 6,998 2 View

Viral co-infection?

Hello everyone. Can someone please give me some indications about the experimental conditions required for a good viral co-infection (Hepatitis B and C virus) in Huh 7.5 cell?

08 March 2016 5,364 2 View

Coronavirus and its impact on peoples life all around the world!

These days coronavirus is everywhere, I mean not only in the contaminated countries all over the globe but also in the news,.. even here in research gate I feel like people are only discussing...

01 January 1970 3,741 3 View

Why meaningful scientfic findings are slowing down?

Science History is a continously changing curve...some periods called the golden age of Science were characterized by several inventations and findings that has contributed in a major way to the...

01 January 1970 2,171 0 View