Iskander Madhi

15 Questions 68 Answers 0 Followers

Questions related from Iskander Madhi

Can you suggest a good software to make graphs for scientific papers?

I am in the process of making graphs for my scientific data and I want to know what is the best software available and most easy to use that you already are familiar with.

20 September 2023 8,199 5 View

Can anyone help me with BD Accuri C6 Software?

I need to figure out a way how to modify the graphs. By modifiying the graphs I mean changing the frame or the style that makes the line of each graph, also, I want to change the filling of each...

14 August 2019 8,558 2 View

Does the hepatoma cell line, HLCZ01, really support HBV and HCV entire life cycle?

Hello everyone, I am really intrested on the mechanisms of virus entry and replication of HBV and HCV in different hepatoma cell lines. I heard about the new established hepatoma cell line,...

12 May 2016 4,232 1 View

HBx detection via Western Blot?

Hello everyone, I am trying to detect HBx protein from Hepatitis B Virus through Western Blot. Knowing that the molecluar size of this protein is 17kD can anyone give me an indication about the...

27 April 2016 6,692 2 View

Core and HBx proteins western blot detection?

Hello everyone, I need to detect core protein (25kD) for HCV and HBx (17kD) from HBV by western blot. I already detected but the band are still not as clear as I want. I used a 12% SDS gel,...

18 April 2016 6,947 4 View

Hbx snd core protein western blot detection?

Hello everyone, I am trying to detect hbx and core protein with western blot, is it better to use a 10% or 12% Sds-polyacrylamide gel? Thank you in advance

05 April 2016 3,064 3 View

HBV and HCV co-infection in Huh 7.5 cells?

Hello everyone, I am starting a co-infection experiment with Hepatitis B virus and Hepatitis C virus in Huh 7.5 cells. I already consult many reviews and I found that three options are possible:...

04 April 2016 496 1 View

HCV and HBV real-time PCR quantification?

Hello everyone, can someone tell me the exact differences in the different parameters (florescent dye, number of standards, quantity of the template...) of the real-time PCR for the HBV and HCV...

31 March 2016 7,088 1 View

Can anyone share methods for HCV RNA detection with PCR?

Can anyone shear with me the (RT-PCR or PCR) conditions for a good detection for the HCV virus. Thank you in advance.

18 March 2016 7,009 2 View

Infection of Huh7.5 cells or HepG2 cells by Hepatitis B virus?

Can aynone, shear with me or give me any indication about the resultat (quantity of viral copies) that I should find in Hep G2 or Huh 7.5 cells infected with a 0.1 MOI of HBV, Taking that the...

18 March 2016 9,837 2 View

Viral co-infection?

Hello everyone. Can someone please give me some indications about the experimental conditions required for a good viral co-infection (Hepatitis B and C virus) in Huh 7.5 cell?

08 March 2016 5,373 2 View

What it the protocol for making a stock of 1.2-mer WT HBV replicon?

31 January 2016 5,164 0 View

Coronavirus and its impact on peoples life all around the world!

These days coronavirus is everywhere, I mean not only in the contaminated countries all over the globe but also in the news,.. even here in research gate I feel like people are only discussing...

01 January 1970 3,753 3 View

Why meaningful scientfic findings are slowing down?

Science History is a continously changing curve...some periods called the golden age of Science were characterized by several inventations and findings that has contributed in a major way to the...

01 January 1970 2,180 0 View

What is the relationship between Autophagy and sleeping?

Is there any possible correlation between Autophagy and our sleeping habits? Is it a reciprocal relationship or just a unidirectional one?

01 January 1970 858 4 View