There are different T2A sequences available although all of them share the same protein sequence. Are they codon optimized according to species?
We have DNAse I stock solution (obtained commercially, still lyophilized, should be dissolved in 10 mL according to supplier) and it has been sitting in a closet way too long (nearly 10 years)....
09 June 2022 2,945 0 View
We want to design antisense oligonucleotides for suppressing the gene of interest. The databases or related literatures found are either old or irrelevant. We are new in this field. Are there ASO...
22 March 2022 5,469 0 View
We are planning to transfect HEK293T cells with plasmid that carries shRNA sequence against the gene of interest, grow a large batch and let them produce shRNAs, and then isolate shRNAs. But the...
10 March 2022 1,781 1 View
We want to inhibit gene expression via shRNA in prostate cancer cell lines and then store them for a while for further experiments (apoptosis, proliferation, qRTPCR etc.). I know that it is...
04 January 2022 5,726 2 View
Cell surface proteins can be specifically biotinylated, but the enrichment of biotinylated proteins is based on streptavidin beads that are used upon cell lysis and release of proteins to a...
17 October 2021 5,661 6 View
We study the primer cell line of the epidermis. To obtain single-cell from the epidermis separated by disease but Dispase is lyophilized. It is 0.05 U/mg. We will use 2.4 U/ml. How should we...
20 April 2021 4,455 2 View
Do we need to have both substrate and sealant materials in melt state at the interface for a strong heat seal?
30 November 2020 1,907 0 View
Can you share any relevant atricles? Can you share the curremnt knowledge in this? Any application examples other than gels / membranes? Thank you
11 September 2019 4,073 1 View
Dear community I would like to ask if you are aware of any tool (Matlab/toolbox, etc.) that calculates the "lag between BOLD time series", as described in the following publications:...
01 November 2018 2,572 2 View
Application of sodium polytungstate is a widely used method for separation of soil aggregates based on their density (eg. Zimmermann et al. 2007). But during the centrifugation process the color...
09 March 2016 873 3 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View