We study the primer cell line of the epidermis. To obtain single-cell from the epidermis separated by disease but Dispase is lyophilized. It is 0.05 U/mg. We will use 2.4 U/ml. How should we dilute it to get this value?
Dear Sevinc, in enzyme units or specific enzyme activity case you need to use certain formulas and in your case it can be achieved by following way;
0.05 * 1 / 2.4 = 0.0208 ml or it means 20.83 ul. So the 1 mg of 0.05 U / mg Dispase would be dissolved in 20.83 ul diluent. If your Dispase vial has total 5 mg of Dispase powder so you can add 20.83 ul * 5 = 104.15 ul. Otherwise, multiply by the value written on its vial.
Conversely, for example you want to prepare lets say 10 ml of 2.4 U / ml Dispase from 0.05 U / mg so now; 10 * 2.4 / 0.05 = 480 mg. So you will take 480 mg from Dispase vial and add 10 ml diluent, which will give you 2.4 U / ml.
So whatever you want to plan just put respective values in the above formulas to achieve your desired target. Hope you get it....
Regards.....
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