In Gaussian, how to calculate energy of a low-spin antiferromagnetically coupled binuclear complex?

Dear colleagues, I would like to ask you for help. I have been trying to calculate the energy of a binuclear Cr(II) organometallic complex, where the Cr(II) atoms are connected via hydride...

08 November 2020 563 2 View

Should I test for HWE in dataset of inbred animals without random mating?

Hi, I am doing a GWAS analysis of european bison's a dog's SNPs. Right now I am doing multiple data clean up, according to previous studies and what I have learned. I was thinking, if it is ok to...

18 March 2020 3,103 3 View

Does anybody know an sientific/empirically paper about psychological requirements in teamsports (e.g. ballsports)?

I am writing my Bachelor thesis about psychological requirements in volleyball. I appreciate any help I could get :) Thank you so much

08 February 2020 5,728 4 View

How to compare the results of variation partitioning for two diferent communities?

12 September 2019 6,578 1 View

How actual physical thickness (AOT) of Si02 can be calculated from effective oxide thickness (EOT) ?

As we all know if the EOT of a device is 3nm that doesn't mean that the actual oxide thickness is 3nm. so my question is how we can calculate the Actual oxide thickness (Physical ) of SiO2 from a...

28 July 2019 3,996 1 View

How can the efficiency of soft annealing on aluminium conductor be controled?

How can the wire producer or the customer control the effectivnes of annealing process to get enough soft wire? Are there some limit values of mechanical properties to test Al-conductor class2...

11 December 2018 4,824 5 View

MiRNA RT-PCR curve from nativ sample (blood isolate) dont match synthetic miRNA PCR curve?

20 April 2017 7,826 2 View

What statistical test to use to find out what taxa significantly differ between several treatments?

20 December 2016 6,444 4 View

No title

when is the counterstaining with haematoxylin good?

31 October 2016 5,499 3 View

V5 His tag N terminal mammalian expression?

Hi, I`m looking for a plasmid to subclone into a mammalian system. It should have V5 and a His tag on N-terminus. Any suggestions?

14 September 2016 2,576 5 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View

What statistical test is required for an analysis on pre-COVID & post-COVID patient improvement after attending a 10-week programme?

Hi all, Myself and my supervisor are looking at carrying out a statistical analysis to see whether there is a difference in patient outcomes for our 10-week pain management programme according to...

10 February 2021 6,622 10 View