Lenka Koptašiková

6 Questions 7 Answers 0 Followers

Questions related from Lenka Koptašiková

Why are plant tissue or green microalgae still green after Osmium Tetroxide staining for EM?

I prepared the plant tissue (small cotyledones of A.thaliana or also marine microalgae Pterosperma cells) for TEM using standard chemical fixation. After osmication (3h for plants, 1h for...

01 December 2023 3,078 0 View

What is the application of Click-iT chemistry in protein detection?

Hello everyone, Is it possible to detect any protein in the cell using click-iT chemistry (Nobel Prize, 2022) in the living cells? Thank you, Lenka

15 October 2023 4,260 1 View

Solidification of Lowicryl-ethanol solution during Progressive Lowering of Temperature close to the samples?

In the biological samples (suspensions or plant tissues) processed by PLT method I experienced creation of gel-like solution close to the sample when I was infiltrating the samples with mixture of...

15 July 2022 3,690 2 View

Dark lines through cells embedded in epoxy resins for TEM?

Dear all, have you experienced dark lines that are localized primarily within the cells (detailed TEM pics.)? Cells were embedded in epon for TEM (but I experienced the same lines in araldite and...

19 March 2019 4,090 4 View

Have you experienced dark spots in formvar-coated TEM grids?

Here is one picture of dark spots. I prepared 1.5% formvar in chloroform and diluted it into 0.5%. After o/n incubation I performed carbon-coating. Could it be the result of insuficient drying or...

01 March 2019 7,513 10 View

"Ghost" mitochondria in TEM?

After GA-osmium fixation of fibroblast I experienced ghost mitochondria in TEM. Have you experienced that the rest of the cell is fixed properly but not for mitochondria? Protocol Fix solution:...

01 January 1970 2,939 0 View