I carried out PCR to amplify my oligo library and digested with a restriction enzyme before cloning into the vector. Prior to cloning I carried out QC using TAPE station. The QC results was completely different from Agarose run. The bands were migrating differently and there are double peaks, that is not evident in Agarose gel (see the attached picture). Agarose gel shows expected size (~265 bp undigested and ~225 bp when digested). Please let me know what causes this anomalies?

(Pictures attached)

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