29 October 2019 1 4K Report

I am planning to do a MPRA assay for ~6000 variants. A friend of mine suggested to use Agilent as it cost $7000 for 100, 000 oligos. I was wondering what other options that I have to get my oligos synthesized?

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Does Anyone Know How to solve this issue in QGIS + SWAT plus modeling?

Traceback (most recent call last): File "peewee.py", line 3237, in execute_sql sqlite3.OperationalError: attempt to write a readonly database During handling of the above exception, another...

03 June 2023 6,465 0 View

Which is more important: domain knowledge or technical proficiency?

Technology has come from the laboratory to society. There are two groups: 1. individuals with technical expertise (Ex; AI, including ML) and limited work experience and domain knowledge and 2....

11 February 2023 4,949 3 View

Looking for a surface water supply data for agricultural purpose in Kerala?

I am looking for Surface water supply data for build a water conjunctive management model in Kerala India.

01 February 2023 7,188 3 View

Dear researchers can you explain what is the best reference software i.e .Mendeley or Zotero?

I want to clarify what is the best referencing software Mendeley or Zotero

15 December 2021 8,707 6 View

How to estimate the extent to which the FE domain should be meshed with explicit elements in Explicit-Implicit Time integration procedure?

I am developing a MATLAB code for shock problem and want to divide the FE domain into explicit and implicit elements (region of domain at vicinity to shock is meshed into explicit elements and...

15 January 2021 2,244 2 View

Why Agarose gel run is very different to TAPE station based QC data?

I carried out PCR to amplify my oligo library and digested with a restriction enzyme before cloning into the vector. Prior to cloning I carried out QC using TAPE station. The QC results was...

12 August 2020 9,985 2 View

How to calculate stress using Electrodermal activity Activity (EDA)?

Currently I am working on a project about measuring stress using EDA. I have seen a formula known as Baevsky Stress Index to calculate stress using Heart Rate Variability. Does anyone know some...

01 October 2019 3,035 4 View

What is the effect of subtended angle of arcs of cylindrical shell panel under UDL loading?

I have coded for static analysis of cylindrical shell panel using shell theory. I have validated the code from the published results which I have found only for shallow shells. However, the same...

18 February 2019 2,283 0 View

How to model the following stochastic programming problem?

The problem in brief is as follows. If (T_1 < T_2) Y = 1 ---------------(1) else Y = 0 ---------------(2) Then, Prob(\sigma Y < C) > \alpha...

15 October 2018 2,190 4 View

Can Tail cuffs used to measure blood pressure in anesthetized mice?

Hi all! Currently I am investigating a hypertensive mouse model and I am planning to use tail cuffs to screen mice, that have undergone recombination, before I proceed with telemetry based blood...

09 August 2018 9,475 6 View

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Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

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