Kushan Gunawardhana

4 Questions 6 Answers 0 Followers

Questions related from Kushan Gunawardhana

Why Agarose gel run is very different to TAPE station based QC data?

I carried out PCR to amplify my oligo library and digested with a restriction enzyme before cloning into the vector. Prior to cloning I carried out QC using TAPE station. The QC results was...

13 August 2020 10,024 2 View

What's the best company to generate oligos for MPRA?

I am planning to do a MPRA assay for ~6000 variants. A friend of mine suggested to use Agilent as it cost $7000 for 100, 000 oligos. I was wondering what other options that I have to get my oligos...

29 October 2019 4,006 1 View

Can Tail cuffs used to measure blood pressure in anesthetized mice?

Hi all! Currently I am investigating a hypertensive mouse model and I am planning to use tail cuffs to screen mice, that have undergone recombination, before I proceed with telemetry based blood...

10 August 2018 9,505 6 View

What is the function of a reporter gene in massively parallel reporter assay?

Hi all, When I was reading on massively parallel reporter assay (MPRA) literature, I found many studies use GFP/Luciferase like reporter genes when they construct the MPRA library. What is the...

23 February 2018 2,977 4 View