Hi all! Currently I am investigating a hypertensive mouse model and I am planning to use tail cuffs to screen mice, that have undergone recombination, before I proceed with telemetry based blood pressure measurements. As expected, tail cuff measurements are highly variable and I am thinking this may be due to the restraining stress during the measurements. Therefore, I am planning on using anesthetized mice to reduce the stress level. Any help or suggestions with these experiments are highly appreciated.
all narcotics have an influence on blood pressure so i would not recommend.
Thank you very much for your input and the suggestions. As Natalia suggested, I keep them three days to acclimate to the procedure, even with that the values are changing. Thanks again for your input
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Hi, well a lot of good advices and considerations. Some precisions : tail cuffs in mice are totally unreliable. So, no need to have any hope using them to sort out your animals. Second anethesia will most of the time drop the pressure, true. I read that flow in the tail will decrease, of course certainly, but what reliable link do you have between flow and pressure ? Strange. You may have a small flow and a very high pressure.
You are welcome.. If you need an anesthesia which does not lower blood pressure, use urethane. But it has other drawbacks.
Hi Renaud and Natalia, Thank you very much for your suggestions! We ultimately decided on telemetry based measurements.