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Hello , I want to do western blot for my protein (Bcl-2 family molecular weight from 10 - 65 KDa , wich percentage of gel acrylamide i prepare? 10 % , 12 % or 15 %? Thanks in advance for your answers
04 May 2017 3,245 5 View
Hi,What factors to consider when deciding between alfa- MEM vs. DMEM-HG or DMEM/F12 when growing placenta derived MSC ? why alfa MEM is more better than DMEM-HG or DMEM/F12 . Thanks for your...
04 May 2017 568 0 View
hello all , to evaluated the adipogenic differentiation , we use oil red o staining , i use 0.3 % or 0.5 % from this solution best regards
04 May 2017 5,985 6 View
hello i collect MSC and i want to do immunofluorescene to detect the surface antigen CD -73 AND CD 90 , it is the first time i want to do flow cytometer . someone has a video or a protocol...
04 May 2017 3,929 5 View
hello all i stain MSC differentiated in adipocyte by oil red o , i read that i must use the isopropanol to do quantifiying , please some one can help me by sending the protocol . thx in advance
04 May 2017 5,901 8 View
hi all ! someone can help me and send me the protocole how can i isolate the MSCs from wharton' jelly umbilical cord human and the protocole of flow cytometry to caracterize the cell marker...
09 October 2016 1,666 0 View
Read 5 answers by scientists with 4 recommendations from their colleagues to the question asked by Linda Scavo on Sep 13, 2016
08 September 2016 620 5 View
i rinse three times after i blok my membrane with BSA-PBS TWEEN ... then i put my primary antibodies with BSA -TBS TWEEN NOT PBS .. U THINK i will have a problem because i use PBS TWEEN AND TBS...
08 September 2016 9,944 0 View
dear collegues , i'm needing your help with the protocol of differentiation of MCS human , i want put to the media 50 ml l-ascorbique ACID concentration 50 micromolar , how can i calculate the...
07 August 2016 7,050 9 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...
25 July 2024 5,001 2 View
"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...
23 July 2024 9,109 0 View
Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...
17 July 2024 2,787 4 View
My protein is a transmembrane protein, therefore i need to construct a transmembrane using CHARMM-GUI. Can someone please suggest me a tutorial for the same.
24 June 2024 5,044 1 View
Hi, may I know I want to fuse my protein with affinity tag for protein purification in Expi293, how can I determine and visualise how does a N or C terminal tag affect my proteins (structure,...
24 June 2024 8,622 6 View
Hello esteemed scientists, I am endeavoring to increase the yield of my recombinant protein. It is a 120 kDa protein tagged with MBP and features three intrinsically disordered regions with pI...
12 June 2024 9,616 8 View
