Can someone help me about oil red o staining ?

More Linda Scavo's questions See All

Can someone help me?

Hello , I want to do western blot for my protein (Bcl-2 family molecular weight from 10 - 65 KDa , wich percentage of gel acrylamide i prepare? 10 % , 12 % or 15 %? Thanks in advance for your answers

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Please i need your help ?

hello all i stain MSC differentiated in adipocyte by oil red o , i read that i must use the isopropanol to do quantifiying , please some one can help me by sending the protocol . thx in advance

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Hi all !! Someone can help me please? Can I block my nitrocellulose membrane with BSA/TBST overnight?

Read 5 answers by scientists with 4 recommendations from their colleagues to the question asked by Linda Scavo on Sep 13, 2016

08 September 2016 620 5 View

Hi all !! Someone can help me please urgently ?

i rinse three times after i blok my membrane with BSA-PBS TWEEN ... then i put my primary antibodies with BSA -TBS TWEEN NOT PBS .. U THINK i will have a problem because i use PBS TWEEN AND TBS...

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Mushtaq Ahmad

http://bio.lonza.com/uploads/tx_mwaxmarketingmaterial/Lonza_ManualsProductInstructions_Oil_Red_O_Stain_for_In_Vitro_Adipogenesis.pdf

follow this one

Linda Scavo

thanks a lot , mushtaq ahmad

Thinhinane Hocine

Good morning Linda,

I personnaly use a 0.5% stock solution, and then for the working solution i mix the parts stock solution with two parts distilled water.

I will send you my protocol by DM

good luck

thanks a lot thinhinane hocine

Sandip Patel

I will send you my protocol. Please see attached file.

Good luck

Thanks sandip