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Questions related from Linda Scavo
Hello , I want to do western blot for my protein (Bcl-2 family molecular weight from 10 - 65 KDa , wich percentage of gel acrylamide i prepare? 10 % , 12 % or 15 %? Thanks in advance for your answers
05 May 2017 3,203 5 View
hello all i stain MSC differentiated in adipocyte by oil red o , i read that i must use the isopropanol to do quantifiying , please some one can help me by sending the protocol . thx in advance
05 May 2017 5,867 8 View
hello all , to evaluated the adipogenic differentiation , we use oil red o staining , i use 0.3 % or 0.5 % from this solution best regards
05 May 2017 5,953 6 View
hello i collect MSC and i want to do immunofluorescene to detect the surface antigen CD -73 AND CD 90 , it is the first time i want to do flow cytometer . someone has a video or a protocol...
05 May 2017 3,899 5 View
Hi,What factors to consider when deciding between alfa- MEM vs. DMEM-HG or DMEM/F12 when growing placenta derived MSC ? why alfa MEM is more better than DMEM-HG or DMEM/F12 . Thanks for your...
05 May 2017 464 0 View
hi all ! someone can help me and send me the protocole how can i isolate the MSCs from wharton' jelly umbilical cord human and the protocole of flow cytometry to caracterize the cell marker...
10 October 2016 1,626 0 View
Read 5 answers by scientists with 4 recommendations from their colleagues to the question asked by Linda Scavo on Sep 13, 2016
09 September 2016 558 5 View
i rinse three times after i blok my membrane with BSA-PBS TWEEN ... then i put my primary antibodies with BSA -TBS TWEEN NOT PBS .. U THINK i will have a problem because i use PBS TWEEN AND TBS...
09 September 2016 9,912 0 View
dear collegues , i'm needing your help with the protocol of differentiation of MCS human , i want put to the media 50 ml l-ascorbique ACID concentration 50 micromolar , how can i calculate the...
08 August 2016 7,012 9 View
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06 June 2016 3,179 0 View
