Does anyone have an old digital 'whole slide' scanner that they can donate to a good cause?

New transplant programme starting in a small African nation, but they will need support and training in the examination of biopsies. A digital 'whole slide' scanner would allow virtual biopsy...

27 November 2019 9,900 1 View

Is transgender a feeling or a desire?

I encounter difficulty in getting the sense of transgender. If it is feeling how does it develop? If it is desire what motivates it? It is important that I should have knowledge on this phenomena...

23 July 2019 5,137 4 View

What are possible substitutes for P2O5 as a desiccant?

I've been using phosphorus pentoxide as the desiccant while drying my enzymes; however, our stockroom has now run out of the chemical. What are other chemicals that can be used as desiccants that...

12 January 2018 8,072 4 View

How to calculate the average correlation across samples in a meta analysis?

Dear researchers, I am conducting a meta analysis which analyses the correlations between personal values and different well-being scales across different studies and by that across different...

26 December 2017 748 6 View

Have a PCR product obtained with degenerate primers, need to clone. Interesed in one band specifically, how do I choose the ratio product:vector?

I am using degenerate primers to amplify a family of genes. My results in an agarose gel are 6 bands (862pb, 632pb, 408pb, 335pb and 226pb). The bands of interest are 408pb and 335pb. What...

27 November 2017 7,346 3 View

Any one tell me. what is the concentration of below the sample buffer 1x or 10x?

14 July 2017 9,607 3 View

Do I need longitudinal data for path analysis?

Dear researchers, I plan to investigate possible causalities of certain psychological constructs and variables like Engagement, Goal attainment, Well-being... Based on correlation analyses and...

09 November 2016 3,681 2 View

What's the most recent Portraits Values Questionnaire version (PVQ-RR?)?

I would appreciate it and be very happy if anybody could tell me which is the most recent Portraits Value Questionnaire (PVQ) based on Schwartz et al. 2012 with 57 items (not PVQ21 or PVQ40)?...

20 March 2016 2,614 8 View

Almost all soil analyses carried in the laboratory are done on 2 mm sieved soil. Can someone please explain why?

04 July 2014 4,221 14 View

In a potted maize experiment, what is the appropriate way to collect leachate? What is the appropriate leachate storage temperature and duration?

l am about to carry out a pot experiment and l need to know the appropriate way to collect leachate, its storage temperature and duration and the appropriate leachate sampling interval/period. l...

10 March 2014 9,902 10 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

No title

23 February 2021 4,014 3 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

How to perform EEG classification of Alzheimer and control groups?

I am doing a project on classification of EEG signals of Alzheimer's disease and control groups. Previously, I extracted the alpha power of the EEG signals and found out the strengths. I want a...

18 February 2021 8,502 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Why is there violent fluctuation of ThT signal in Amyloid beta aggregation?

Dear all, I am currently working on amyloid beta aggregation. In ThT monitered aggregation assay, I have already obeserved an increase in ThT signal, indicating the aggregation of amyloid beta....

15 February 2021 4,852 2 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View