Reeteka Sud

4 Questions 10 Answers 0 Followers

Questions related from Reeteka Sud

Cell number for 1mg protein?

We need to isolate 1mg protein, from ipsc-derived cortical neurons. About how many cells should I have, to get this protein amount from these cells?

18 December 2020 3,154 1 View

What is the lifespan of differentiated SH-SY5Y?

I am looking for info on the longest time that differentiated SH-SY5Y neuroblastoma have been cultured.

19 August 2014 4,449 5 View

>20deg differential between Tm values of F- & R- primers - how to set annealing temperature for PCR?

F primer has Tm around 70deg, while R-primer's Tm is 43deg. I am planning on doing a gradient PCR- not sure what is the lowest value to set for annealing, without risking non-specific...

12 June 2013 1,772 6 View

Experiencing problems sequencing plasmid after cloning: see sharp peaks at the beginning; signal

Trying to sequence pCDNA vector after cloning in multiple inserts. Sequencing primers align

05 June 2013 1,345 22 View