Reeteka Sud

3 Questions 9 Answers 0 Followers

Questions related from Reeteka Sud

Cell number for 1mg protein?

We need to isolate 1mg protein, from ipsc-derived cortical neurons. About how many cells should I have, to get this protein amount from these cells?

18 December 2020 3,150 1 View

>20deg differential between Tm values of F- & R- primers - how to set annealing temperature for PCR?

F primer has Tm around 70deg, while R-primer's Tm is 43deg. I am planning on doing a gradient PCR- not sure what is the lowest value to set for annealing, without risking non-specific...

12 June 2013 1,768 6 View

Experiencing problems sequencing plasmid after cloning: see sharp peaks at the beginning; signal

Trying to sequence pCDNA vector after cloning in multiple inserts. Sequencing primers align

05 June 2013 1,343 22 View