Hi. It is because you do not want to rapidly change the osmotic pressure and cause influx of water into the cells as this will cause cell lysis
Yes I agree with Cheryl; and also the cells after thawing should be in a fluid with the appropriate ions and ionic strength similar to what obtains in the human blood where they thrive during an infection
Cheryl is correct. The cells are often frozen in glycerol/glycerolyte and the stepwise addition of NaCl solutions from 12% to 1.6% then 0.9% prevents the rapid entry of water to the cells, and thus prevents lysis. We have some robust protocols on out website, which work well with most frozen isolates. Don't forget ABO blood group when thawing and setting up cultures using human serum.
www.malariaresearch.eu
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