Plant tissue culture, media preparation, commercial scale production of in vitro plants
We use Millipore water. But, double distilled water should be ok for plant tissue culture purpose too, just remember to autoclave your medium.
Any contamination free water will serve the purpose. Some of the salts in RO water may affect growth performance of TC initiation. Millipore water will be the best to use.
Sharma et al. (2013) reported that there was 85% reduction in cost of media for plant caulogenesis by using inexpensive carbon source, water source and gelling agent. Laboratory reagent grade sucrose was replaced by locally available commercial sugar (market sugar and sugar cubes) as carbon source, bacteriological grade agar by isabgol (Plantago ovata), sodium alginate, starch as gelling agent and distilled water, tap water and packaged drinking water as a water source. Based upon cost analysis the use of isabgoal as a gelling agent, use of sugar cubes and packaged drinking water for preparation of media can be used to boost the caulogenesis in Stevia rebaudiana.
Sharma, V., Singh, I. and Sharma, S. 2013. Formulation of medium with low cost options for in vitro caulogenesis in ethnomedical herb Stevia Rebaudiana. Trends in Biotechnology Research Volume 2, Issue 1 : 36-40.
Hi Asha,
I attached a booklet describing the detailed in and out of the 'Research Water'. It was published by the National Institutes of Health (USA 2013). It should offer you a great insight of all kinds of water used for research.
Millipore utilizes the RO technology for water purification. If you are using Millipore water, you are basically using RO water. Hope this helps.
Distilled water would be fine, but keep in mind even distilled water "properties" or quality may vary from place to place. So, you better give a try first, then go on.
In scientific researches, it must be used high purified water regardless of purification methods, but in commercial plant tissue culture, there are 3 cases,first : all the stock solutions from any chemicals (PGRs,....) must be prepared from high purified water. second, for media preparation, you can use distilled water or "in some tolerant plants, in some commercial labs" tap water according to quality of water to decrease the cost, whereas the medium has more than 90% water. third: for sterilization of explants, you can use distilled water besides tap water contamination-free except in some cases ( sensitive plants, presence of limiting growth factors,...). finally I agree with Luis Carlos Ramos, you better give a try first, then go on. regards
Hello Asha
I agree with Metwally. We run a commercial propagation laboratory and use distilled water for stock solutions and hormone preparations, collected tank water which is run through a 5 filter plus uv light for media preparation, this water also is used for washing plants prior to sterilisation and also for the purpose of added sterilants for cleaning.
The filters and uv light remove particles which collect in the water from the air and the UV renders bacteria useless. We use a household setup which we then test with a commercial testing agency to see if the product was completing the action required. The next system was installation of millipore which was beyond our budget but does give excellent water quality. The upgrade on this is RO which is the ultimate but again quite an investment
We never use water for public consumption as aluminium, fluoride, chlorine is used plus agents unknown which may react with your media elements or plants
Good luck with your trials
We used Millipore purification systems in chemistry analytical labs. Millipore had both carbon filters to remove organic substances and RO filters to remove dissolved inorganic substances. Our Milli-Q system probably also had a UV lamp for sterilization. RO and distillation alone do not remove all organics. I do not know, however, what standard(s) of water purity are necessary for your tissue culture work.
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