What should I do if Agrobacterium keep on growing on the selection medium? Should i increase antibiotic concentration?

Currently i am in the second cycle of antibiotic selection. I use Hygromycin 20mg/ml stock and Carbenicillin 400mg/ml. Any suggestion so that i can eliminate Agrobacterium without causing albinism...

27 April 2015 5,768 40 View