I measured reducing sugar using the DNS method, but when I removed the test tubes from the water bath after incubation and left them to cool, I noticed that the colour had vanished. Please solve this problem.
Kindly confirm how to distinguish glia cells and neurons using cresyl violet staining. Also, using cresyl violet nissl staining how to identify the apoptotic neurons ? Does this stain stains...
18 July 2024 2,420 0 View
Kindly confirm can we do MTT assay for quantification of apoptosis. Is MTT Assay a valid assay to quantify apoptotic cells ? What about LDH assay ?..how is it better for apoptosis quantification...
08 July 2024 5,482 2 View
I am determining the mitochondrial membrane potential of mitochondria (isolated from rat brain tissue) using Rhodamine 123 dye. In my experimental procedure, i take the reading of...
08 May 2024 5,081 1 View
Can u plz tell me how to solve this equation using BEM?
05 February 2024 3,635 1 View
Kindly confirm: Can we take PTZ (Pentylenetetrazole) model as model of Status Epilepticus and clinically co-relate with human MTLE its findings ? If we want to study acute changes of...
08 January 2024 7,651 3 View
I am working on animal model of Epilepsy. I wanted to ask if it is ethically allowed to perform neurobehavioural tests (T-maze, rota-rod etc) on my acute model rats (maximum survival days: 72...
01 January 2024 3,300 0 View
Dear all I have been pondering over this for a while. Please help me. Thanks and best
01 January 2024 9,402 6 View
Could anyone kindly provide the derivation of the Confinement loss equation for hollow core fiber, or recommend a book or a paper where the derivation is explained? Thanks in advance.
19 October 2023 6,564 0 View
Being an activator of Mitochondrial calcium uniporter, how can spermine (polyamine) decrease calcium concentration in mitochondria ? In my experiments, spermine is decreasing calcium concentration...
19 June 2023 4,739 0 View
Kindly confirm how can we interpret data of delta Ct in RT-PCR Results? We have just control tissue, not any test sample; thereby delta delta Ct values can't be find out. We hv just delta Ct...
04 December 2022 3,059 2 View
I have a response variable called skin yellowness, which I will measure via a scored color chart, whereby 1 is pale yellow and 15 is orange. I'm not sure if this counts as an ordinal variable,...
11 August 2024 4,793 1 View
is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it
06 August 2024 947 1 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...
30 July 2024 6,794 0 View
Using SPSS, I made my edits in one bar chart — e.g., font type and size, hiding grid lines, and colours, namely: blue, orange, green, purple, and grey for 5 bars, respectively, etc. — and I saved...
29 July 2024 1,981 1 View
Genome-wide association study (GWAS) Continuous traits: eg. Height Discrete traits: eg. Eye color
28 July 2024 286 0 View
Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...
26 July 2024 2,136 0 View
I used Aqua regia to clean Au nanoparticles in the used glassware. But for some reasons, it is not heating up upon mixing. The color did change from no color to light orange. The Au NPs on the...
25 July 2024 4,390 0 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
Despite not having cells in the media, I am getting purple color. I have tried many troubleshooting methods, varying media types, and even different MTTs from different companies to figure out the...
21 July 2024 9,914 1 View