In some studies to measure cytokines in the supernatant for the culture of spleen mononuclear cells, after a while, the suspensions cells are separated from sticky cells and cultivated on a separate plate and the supernatant were collected from suspensions cells. I do not know the reason for this and why do they separate sticky cells?
Monocytes are the sticky cells.
The reason for separating them would depend on the aim of the study. Basically, removal of monocytes from the rest of the cells would establish their importance, or lack thereof, in subsequent studies.
Usually, telomerase-negative differentiated cells and progenitor cells make their own matrix for attachment and will attach to the matrix they synthesize on the plate surface in 18-24 hours. Alternatively, telomerase-positive stem cells do not synthesize their own matrix and unless their matrix is provided for them they will not attach to the plate surface, no matter how long they are in culture.
When culturing bone-marrow derived macrophage, we put all stem cells into the plate for one day with cytokine. After one day we remove the suspending cells and keep culturing the attached cells to get a pure macrophage population since the suspending cells may turn into dendritic cells. Maybe in spleen it's the same reason.
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