Can RNApolymerase bind to RNA?

Given that the core enzyme tends to DNA in the cell, because of the electrostatic force. Why is there no such tendency between RNApolymerase 2 and RNA?

14 December 2020 2,708 5 View

What are the methods used to amplify the voltage received from two electrodes used in a micro channel to count micro and nano particles?

i'm recently on counting of micro & nano particle by methods like:impedance spectroscopy And I want to get a stronger voltage from my electrodes Who can give me a solution in this matter?

30 June 2020 6,963 8 View

I want to compare the difference of immune system in patient with mild and sever symptom.who should i define the case and control group?

Is it enough to compare people who needed to be hospitalized with people who did not? , or should the age and duration of the illness be considered?

26 May 2020 2,829 2 View

Why the covid-virus infection is very rare in children compare to young and adult people?

As we know the immune system in both children and adult is impaired. The question is that why the covid-virus infection is very rare in children compare to young and adult people

03 April 2020 9,934 11 View

Why do we have differences in numerical and experimental models in the damaged plastic model?

Considering the start of the cracks in the middle of the sample and the acts boundary conditions on both sides of the strain stress curve for composite It is linearly drawn up to the point of...

13 February 2019 2,176 2 View

What is difference between therapeutic and prophylactic group in research ?

I want to evaluate the therapeutic and prophylactic of a drug on RA model in rat. when i should started to administration of drug in therapeutic and prophylactic group? in some studies they...

23 March 2018 3,708 4 View

In known group comparison/differences technique, what is the sample size for each group?

I need to use the known group differences to check the validity of the constructs as part of developing a new instrument. The instrument has seven first-order constructs and one second-order...

03 March 2018 4,179 2 View

Which spleen mononuclear cells in culture plate attached to the bottom plate?

In some studies to measure cytokines in the supernatant for the culture of spleen mononuclear cells, after a while, the suspensions cells are separated from sticky cells and cultivated on a...

01 February 2018 5,035 3 View

How to make crude oil in the laboratory?

Hello How to make crude oil by combining different materials, such as gasoline? Also, can i determine API gravity (American Petroleum Institute) for this Crude oil?

07 January 2018 1,899 6 View

Can anyone help with a problem with dose and prepration of Streptozotocin to induce diabetes in Wistar rats?

01 December 2017 4,408 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View