Hi everyone
I am working on MCF7 cell for anti-cancer purpose and I want to prepare the drug (secondary metabolites extracted from Aspergillus niger but these compounds didn’t identify yet) and I already downloaded my compounds with silver nanoparticles
My first question is which solvent is better with these compounds with nanoparticles is DMSO or Ethanol or FBS? or PPS And I want the exactly preparation method how i calculate the the quantities of compound powder and the quantity of solvent all these details
my second question is how exactly prepared like if there is centrifuge or vortex or filter (i want details )
Thank you