Which primers sets(Sense two sets) Antisense(Two sets) I should use in conventional PCR? Two sense and two antisense primers what does it means?

Dear Researchers, I have read an article and there are two set of primer pairs. Sense primers and antisense primers. The paragraph from the article is as [ The partial DNA-dependent RNA...

31 December 2019 7,502 7 View

Can anyone help in SOD activity buffers?

In 3ml, how can I make all these buffers, I have read this paper for SOD activity, Please find below [The 3 ml reaction mixture contained 50 mM potassium phosphate buffer (pH 7.0), 0.5 mM...

06 July 2019 3,610 6 View

Is there any easy method to get Jasmonic acids from wheat plants?

Dear All, I need to check the growth promoting effects of some bacterial antagonists. I want to obtain the jasmonic acid and other growth promoting hormones. Then I want to do LCMS. Any step by...

05 June 2019 2,520 4 View

How to prepare solution for GC-MS/LC-MS/HPLC from the barley root exudates/leaf extracts?

I want to check some metabolites from the root exudates or leaf extracts. Than I want to check the metabolites concentration like Jasmonic acid, via GC-MS, LC-MS or HPLC. Can anyone explain me...

03 April 2019 3,325 5 View

Can anyone explain PERMANOVA pairwise results?

Dear All, Can anyone explain the pairwise permanova for the group 1 and Group 2. The data is given as below: Group 1 Group 2 Sample size Permutations pseudo-F p-value q-value A C 12 999...

11 December 2018 5,320 0 View

How can I count conidia on the PDA petriplates incubated for two weeks with haemocytomer?

I have a haemocytometer with 25 major grids and each grid is divided into 16 small grids, The dept is 0.1mm. I put 10 ml ddH20 in petriplates and rub to get spores. I take 10ul from that 10 ml...

01 February 2018 2,594 1 View

What is the definition of the strains in terms of fungi?

Dear All, I have read several papers like these: twelve strains of fungus Trichoderma harzianum were evaluated against Phytopathogen. So in these case what would be the definition of strains?

10 November 2017 6,147 10 View

Can someone recommend a proper procedure for extractions of antibiotics and peptides from the fungi?

Dear All, I want to extract antibiotics and peptides from a fungi. Is there any proper procedure to extract antibiotics from the fungi.

10 November 2017 6,618 3 View

Could not get a good and clear band in the Gell electrophoresis?

I run the Gell for DNA of some of samples. However some DNA bands were thinner some were more thicker in size and some of them were like smear from top to bottom. What could be the reason?

10 November 2017 9,762 4 View

How can I measure the percent inhibition of a plant pathogen caused by bi-control fungus?

Dear All, I have cultured two fungi one 9cm petri plates. The growth of biocontrol agent is very slow but it has potential to parasitize a plant pathogen. I want to measure the percent inhibition...

10 November 2017 4,934 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View