Dear Frederico,

check Rob Van 't Hof on research gate. He provides a plugin for ImageJ. http://www.bone-abstracts.org/ba/0001/ba0001PP210.htm

Another software is osteomeasure. http://www.osteometrics.com/index.htm

Consider that you need an experienced environment to generate high quality sections and reliable evaluation.

All the best,

Jirko

Hi Frederico,

I am using Prof. Rob van't Hof's sofware and is working absolutely fine. You can obtain all the necessary bone histomorphometric results for static, cellular and dynamic parameters very quickly, easily and reliably. Highly recommended.

Best wishes,

John

Hi, Frederico,

I have been using Osteomeasure for more than 20 year. The software is capable to do all the basic and some advanced histomorphometry analysis, and meet the publication requirements and high quality. If you may need to do more image analysis, ImageJ would be a good one.

Best,

Yi-Xian Qin

Dear Frederico,

If you want to try my software for Histomorphometry, I've got a web page for it (almost finished) that has links to download pages and some help. also for how to do the histology part. You can find it at :

http://www.liv.ac.uk/ageing-and-chronic-disease/research/imaging/bone-hist/

Alternatively you can download things from my researchgate pages.

best wishes,

Rob van 't Hof

thank you to every one, expecially to Prof. Van 't Hof. Now I have to submit the several alternatives to my chief and my patologist for the acquisition part!

We used BIOQUANT.

free software (Image J 1.48 v, Wayne Rasband, National Institute of Health, USA.) is available and easy to use.

Have you any experience with Zen by Zeiss for histomorphometry?

Zen or AxioVision is the image analysis platform from Zeiss.

AxioVision is a superior software. You have the possibility to establish custom made  quantifications. The disadvantage is you need to buy the basic software plus required modules. This all requires several thousand of euros. I would recommend AxioVision only for an experienced image analyst and a lab that focuses on such issue.

A comment. I think your issue is not a problem of software - you have low cost tools available (e.g. Rob). Please make sure that you  have an expert in bone histology and histomorphometry along side!

best,

jirko

thank you for your comment. The fact is that I'm looking for a sw that can match with my microscope, camera, suitable for a common histomorphometric analysis. 

I agree with Jirko. Once you have a good image of your sections, you can use that in any piece of image analysis software. My images are produced on a motorised Zeiss microscope using Axiovision for image capture. I save the images and analyse in my (ImageJ based) software. The only part of Axiovision's Image analysis modules I use is for image stitching and extended depth of field processing (bone sections are rarely fully flat). As far as I'm aware, Zeiss made the Zen datafiles compatible with ImageJ/Fiji.

The really important bit here is "good image". Doing proper bone histology is not that straightforward. If the expertise for processing, sectioning and staining of bone (ideally undecalcified, methacrylate embedded) is not available in your lab, I would suggest getting training in a lab that does this routinely. 

By the way, what kind of bone histomorphometry parameters are you interested in? Resorption, formation (calcein labels), mineralisation?

best wishes,

Rob

thank you for your good tips!  right now I'm organizing the lab to analyze sample for bone regeneration (anminal and human model) and for bone harvesting before implant placement. I'll need parameters such as newly formed bone, residual graft particles, empty spaces / bone marrow, soft tissues..etc

BioQuant

thank you