Hi, I read the article Fluorescence of Bone, Nature 1965. And they talk about preparation of the bone that have been given in a preceding article. But they don't say which one. I think it's Photoelectric Effects in Human Bone, but I'm not sure. And besides I can't get that article from free. Can anyone help me?
Hi, I have attached the previous paper which is - as you say - on photoelectric effects. I once tried to look at intact bone and can tell you that you have to be very careful to avoid parasitic light since there is so much scattering. I had to place an interference filter in line with the excitation to "clean up" the exciting light. I was exciting at 280nm though and at that wavelength my system was particularly prone to parasitic light or "Rayleigh ghosts".
Good luck
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