Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

In order to simulate a DWC in steady state, how to put the unit operations in the flowsheet of any process simulator?

14 January 2021 3,938 6 View

Which is the best bibliography to search for experimental data of process engineering?

16 December 2020 7,894 2 View

How to set Decouplers in control systems?

09 December 2020 1,409 5 View

Is there a MRI/DTI public database from epileptic patients?

I am looking for DTI/MRI data from epileptic patients for a MSc thesis. Regards

19 November 2020 5,435 3 View

How to built a simulation of a chemical process?

12 September 2020 7,685 10 View

How to recode a short essay style question so that I can use it in SPSS?

Hi everyone, I am conducting an experiment for my dissertation. The manipulation I used consisted in asking people to recall and write down a particular episode in which they felt they had or had...

26 July 2020 6,540 1 View

Is it wrong to measure the mediating variable after the independent variable?

I am conducting an experiment for my dissertation. I want to put the mediator after the independent variable, in the survey flow. However, my supervisor told me that it is wrong to do so. The...

19 July 2020 5,883 5 View

Can the temperature/oxidation state alter the lifetime of an electronic transition?

Good Morning. Iv'e made Fe K-edge XAS measurements on a mineral with Fe3+ and Fe2+ atoms, looking for the pre-edge feature and using gaussian curves to fit the pre-edge assuming 4 quadrupole...

14 July 2020 8,092 4 View

Does Autodock Crankpep's parameter file contain data for Zn?

I'm trying to dock a zinc complex, but when I tried to prepare the ligand, I get an error saying that there are no Gasteiger parameters available for it. I know you can add atom types in Autodock...

29 June 2020 2,031 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View