How to produce a PEDOT:PSS based gel of 30% hydrogen peroxide?

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What are the most frequent typologies used in research in the field of industrial design?

How are industrial design research designs generally configured? What methodologies do they generally use? The idea is to have on hand a repertoire of methods and frequent actions for research in...

08 May 2024 7,666 2 View

What is my ResearchGate code?

what is my ResearchGate code?

05 May 2024 9,749 2 View

SSO oxygen buffer calculation?

I'm searching the parameters and formula to calculate the T and P dependent sulfide sulfur oxygen buffer (SSO) curve. I'd like to add the curve to diagrams where I already added FMQ and NNO Thank You

20 April 2024 4,348 0 View

SARSCOSPHERES - CANINE OSTEOSARCOMA, good protocol ?

Dear all, did any of you already try to obtain sarcospheres from a continuous cell line of a canine osteosarcoma ? Do you have some nice protocol to suggest (if it is published we are glad to cite...

25 February 2024 4,579 0 View

Chip-qPCR alternatives for proteins bound to non-integrating plasmid DNA?

Was wondering if anyone had used any other methods besides ChIP-qPCR to analyze proteins bound to intercellular transgenes. Was thinking of an approach like DiMeLo-seq or other newer strategies....

24 January 2024 5,346 0 View

Difficulties forming collagen hydrogel?

Hi, I am attempting to prepare collagen hydrogels using Corning® Collagen I, Rat Tail (high concentration), which is supplied in liquid form in 0.02 N acetic acid. My objective is to create a...

23 January 2024 8,513 1 View

What are the necessary components to build an anode of a zinc-based redox flow battery with a flow path? In what order should they be inserted?

I would like to attempt to assess the effects of a specific flow path on the anodic part (zinc), but I do not have a clear understanding of all the necessary elements to construct the cell and...

15 November 2023 4,379 0 View

What are the Items Numbers of the NEO PI R questionnaire loading for the Openness facet?

Hello, I am searching for the items that loads for the Openness facet of the NEO PI questionnaire. Can someone just share the items numbers? I have already the items list but cannot know which...

07 September 2023 7,826 1 View

Culturing monocyte derived macrophages?

Hi, I'm trying culturing monocyte, isolated from PBMC's to differentiate them in macrophages. I obtained a buffy coat from a healthy donor, I freezed most of cells in freezing solution...

28 May 2023 9,380 4 View

Different bands after colony pcr at Tapestation. Could anyone help?

I did a ligation between a 9kb vector and a library-pool of inserts 97 base pairs long. I tried three different ratio: 2:1, 1:1 , 1:2 and I need to avoid insert concatamers. After...

24 March 2023 8,443 5 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

What is the ionic radius of Hydrogen anion? And how to know the exact ionic radii of the elements?

I'm trying to calculate the tolerance factors of the hydride perovskite and the value calculated by me and the reported values are contradictory. Need more clarity in using the correct ionic radii...

01 August 2024 2,614 1 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View

How to make the concentration measurements for the hydrogen absorption/desorption in the metal hydride?

Hello Everyone! I am trying to create a metal hydride system where I am trying to create a temperature control with the emphasis on measuring the concentration of the hydrogen with the hydrogen...

29 July 2024 7,577 2 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View