Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

In order to simulate a DWC in steady state, how to put the unit operations in the flowsheet of any process simulator?

14 January 2021 3,938 6 View

Which is the best bibliography to search for experimental data of process engineering?

16 December 2020 7,894 2 View

How to set Decouplers in control systems?

09 December 2020 1,409 5 View

Is there a MRI/DTI public database from epileptic patients?

I am looking for DTI/MRI data from epileptic patients for a MSc thesis. Regards

19 November 2020 5,435 3 View

How to built a simulation of a chemical process?

12 September 2020 7,685 10 View

How to recode a short essay style question so that I can use it in SPSS?

Hi everyone, I am conducting an experiment for my dissertation. The manipulation I used consisted in asking people to recall and write down a particular episode in which they felt they had or had...

26 July 2020 6,540 1 View

Is it wrong to measure the mediating variable after the independent variable?

I am conducting an experiment for my dissertation. I want to put the mediator after the independent variable, in the survey flow. However, my supervisor told me that it is wrong to do so. The...

19 July 2020 5,883 5 View

Can the temperature/oxidation state alter the lifetime of an electronic transition?

Good Morning. Iv'e made Fe K-edge XAS measurements on a mineral with Fe3+ and Fe2+ atoms, looking for the pre-edge feature and using gaussian curves to fit the pre-edge assuming 4 quadrupole...

14 July 2020 8,092 4 View

Does Autodock Crankpep's parameter file contain data for Zn?

I'm trying to dock a zinc complex, but when I tried to prepare the ligand, I get an error saying that there are no Gasteiger parameters available for it. I know you can add atom types in Autodock...

29 June 2020 2,031 2 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software?

Hi every one Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software? Biogas fuel is not available in the options thanks

23 February 2021 6,518 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View