I search for society that make gut microbiom analysis of fishes using metabarcoding like method.
Is that someone already subcontracted molecular steps (DNA extraction to HTS sequencing) to private society for microbiom analysis?
Thank Mariel for the contacts!
I have a list of more than 400 taxa (species level) of fish for which I want retrieve Genbank sequences with (complete or partial) 12S and COI gene. I can download each taxa sequences for each...
10 November 2019 3,792 2 View
I want to use the function geom_bar on my data that look like that: ID Lenght facies rad1 8 rad placou1 12 placou rap1 24 ...
04 May 2017 5,440 5 View
It's a basic problem but I have never done that before. I have 10 species characterized by 6 ordinale variables. How can I measure the "ecological distance" between these species?
06 July 2014 4,428 7 View
Using the TruSeq® Nano DNA, I missed my first Illumina bank (no DNA with bioanalyser). Can you share your experience, it can help me, because I don't know which step I missed.....
06 July 2014 2,286 3 View
After electrophoretic migration of my PCR products, I cut my gel band. Then, I have purified my DNA using the Qiaquick gel extraction kit. After nanodrop, I obtained a correct DNA yield (about...
02 March 2014 7,917 36 View
I plan to inhibit the amplification of my DNA predator during faeces analysis using generalist PCR primer for prey DNA analysis. I have already design modified oligonucleotide (C3 spacer), and now...
01 February 2014 6,005 0 View
Recently, scientific consider different "kind" of parrallel evolution. Recent review states that it can refer to phenotypic parrallel evolution (like a "convergence"), or genetic parrallel...
31 December 2013 8,568 8 View
The use of universal primer to amplify a wide range of prey from predator gut content may amplify also the predator DNA. Thus, predator-specific primer exclusion or predator-specific elongation...
31 December 2013 731 6 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
28 July 2024 7,983 2 View
Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...
26 July 2024 2,136 0 View
Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
25 July 2024 4,927 3 View
Using INTRON's DNA-spin Plasmid DNA purification kit to extract plasmids from specific E.Coli strains, my team and I have encountered numerous plasmid dimerizations that lead to over-weighted...
23 July 2024 3,490 6 View