How to download sequences from Genbang using (long) taxonomic list ?

I have a list of more than 400 taxa (species level) of fish for which I want retrieve Genbank sequences with (complete or partial) 12S and COI gene. I can download each taxa sequences for each...

10 November 2019 3,703 2 View

Can someone help me with the R function of ggplot, the geom_bar?

04 May 2017 5,349 5 View

What is the best method to assess the distance between species ecology?

It's a basic problem but I have never done that before. I have 10 species characterized by 6 ordinale variables. How can I measure the "ecological distance" between these species?

06 July 2014 4,342 7 View

Has anyone missed, then succeeded a DNA bank for Illumina sequencing?

06 July 2014 2,185 3 View

Has anybody used PCR product from agarose gel band for cloning DNA?

After electrophoretic migration of my PCR products, I cut my gel band. Then, I have purified my DNA using the Qiaquick gel extraction kit. After nanodrop, I obtained a correct DNA yield (about...

02 March 2014 7,816 36 View

Does someone have experience in the design of Peptide Nucleic Acids (PNA) for blocking PCR elongation?

I plan to inhibit the amplification of my DNA predator during faeces analysis using generalist PCR primer for prey DNA analysis. I have already design modified oligonucleotide (C3 spacer), and now...

01 February 2014 5,905 0 View

What does Parrallel evolution mean?

Recently, scientific consider different "kind" of parrallel evolution. Recent review states that it can refer to phenotypic parrallel evolution (like a "convergence"), or genetic parrallel...

31 December 2013 8,484 8 View

Is the use of polymerase without exonuclease activity vital for the use of blocking primer or elongation arrest nucleotide?

The use of universal primer to amplify a wide range of prey from predator gut content may amplify also the predator DNA. Thus, predator-specific primer exclusion or predator-specific elongation...

31 December 2013 651 6 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

No title

17 February 2021 4,217 3 View