Emmanuel Corse

9 Questions 27 Answers 0 Followers

Questions related from Emmanuel Corse

How to download sequences from Genbang using (long) taxonomic list ?

I have a list of more than 400 taxa (species level) of fish for which I want retrieve Genbank sequences with (complete or partial) 12S and COI gene. I can download each taxa sequences for each...

11 November 2019 3,741 2 View

Which society perform Microbiom analysis of animals?

I search for society that make gut microbiom analysis of fishes using metabarcoding like method. Is that someone already subcontracted molecular steps (DNA extraction to HTS sequencing) to...

08 August 2017 3,229 1 View

Can someone help me with the R function of ggplot, the geom_bar?

I want to use the function geom_bar on my data that look like that: ID Lenght facies rad1 8 rad placou1 12 placou rap1 24 ...

05 May 2017 5,392 5 View

What is the best method to assess the distance between species ecology?

It's a basic problem but I have never done that before. I have 10 species characterized by 6 ordinale variables. How can I measure the "ecological distance" between these species?

07 July 2014 4,381 7 View

Has anyone missed, then succeeded a DNA bank for Illumina sequencing?

Using the TruSeq® Nano DNA, I missed my first Illumina bank (no DNA with bioanalyser). Can you share your experience, it can help me, because I don't know which step I missed.....

07 July 2014 2,225 3 View

Has anybody used PCR product from agarose gel band for cloning DNA?

After electrophoretic migration of my PCR products, I cut my gel band. Then, I have purified my DNA using the Qiaquick gel extraction kit. After nanodrop, I obtained a correct DNA yield (about...

03 March 2014 7,857 36 View

Does someone have experience in the design of Peptide Nucleic Acids (PNA) for blocking PCR elongation?

I plan to inhibit the amplification of my DNA predator during faeces analysis using generalist PCR primer for prey DNA analysis. I have already design modified oligonucleotide (C3 spacer), and now...

02 February 2014 5,943 0 View

What does Parrallel evolution mean?

Recently, scientific consider different "kind" of parrallel evolution. Recent review states that it can refer to phenotypic parrallel evolution (like a "convergence"), or genetic parrallel...

01 January 2014 8,522 8 View

Is the use of polymerase without exonuclease activity vital for the use of blocking primer or elongation arrest nucleotide?

The use of universal primer to amplify a wide range of prey from predator gut content may amplify also the predator DNA. Thus, predator-specific primer exclusion or predator-specific elongation...

01 January 2014 688 6 View