I came to know that certain hippocampal cell lines don't express estrogen receptors (such as HT22). I was thinking of working on rodent cell line SCR022, but no information about the presence of estrogen receptors in it. I would really appreciate if you could help me on it. Thanks.
HT-22 (mouse hippocampal neurons) seems to be capable of expressing estrogen receptors as evidenced by:
"In order to obtain data regarding the interaction between RyR and ERβ, we examined co-localization patterns of both proteins in intracellular compartments in the neuronal cell line HT-22. While initially thought to be free of functional endogenous ERs [45], the HT-22 cells were found in recent studies to express both endogenous ERβ [30] and ERα [46]".
ref: Rybalchenko V, Grillo MA, Gastinger MJ, Rybalchenko N, Payne AJ, Koulen P. The unliganded long isoform of estrogen receptor beta stimulates brain ryanodine receptor single channel activity alongside with cytosolic Ca2+. J Recept Signal Transduct Res. 2009 Dec;29(6):326-41. doi: 10.3109/10799890903295168.
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Affiliation: SOFA (Sarcopenia & Osteoporosis Fraction in Ageing)
Why can't primary cultures of hippocampal cells be used? Cell lines should be the last resort.
PC 12 cell line has been successively transfected by ER alpha and respond to oestrogen see the paper by Ferriere et al published in PLoS 1 this tear
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